Receptor construct

Maximal binding
(% over basal at 100µM L-Glu)

Emax
[% of  wildtype]

Wild type

198 ± 21 (6)

100

Double cysteine mutants

   

C754A

229 ± 15 (3)

116

C765A

258 ± 10 (3)

130

C793A

122 ± 6 (4)

62

Single cysteine mutants

   

C754A/C765A

134 ± 11 (5)

68

C765A/C793A

6 ± 2 (3)

3

C754A/C793A

99 ± 26 (2)

50

Cys-less mutant

   

C754A/C765A/C793A

94 ± 31 (2)

47

Table 2: Comparison of GTP-Eu binding in the membranes from wild-type and cysteine mutants. Data is represented as mean ± SEM (number of independent experiments). Emax is the relative activity of mutants as compared to wild-type.