Developmental stages DNJ (μg/ per silkworm) Developmental stages DNJ (μg/per silkworm) newly hatched larvae 0.00 a 1st day of instar V 468.62 ± 13.53 i molting larvae of instar I 3.28 ± 0.56 a 2nd day of instar V 710.34 ± 15.41 k molted larvae of instar II 2.91 ± 0.38 a 3rd day of instar V 1499.39 ± 20.13 m molting larvae of instar II 19.12 ± 1.22 b 4th day of instar V 1512.46 ± 25.68 n molted larvae of instar III 19.23 ± 1.09 b 5th day of instar V 990.00 ± 6.85 l molting larvae of instar III 61.30 ± 2.87 e 6th day of instar V 484.55 ±12.66 ij molted larvae of instar IV 80.63 ± 3.49 g matured larvae 68.51 ± 3.01 f molting larvae of instar IV 393.36 ± 10.26 h 1st day of cocooning 39.71 ± 1.42 d molted larvae of instar V 392.72 ± 12.45 h 2nd day of cocooning 26.35 ± 0.98 c

Note: Means without the same letters were considered significant, tested by one-way ANOVA and Fisher’s LSD, p ≤ 0.05.

Table 3: DNJ content of individual silkworm during different developmental stages.