| Parameters |
|
% RSD |
| Chromatographic Condition |
Variation |
PRZ |
AML |
DIL |
VER |
| Mobile phase composition |
MeOH:H2O:ACN (50:40:10) |
0.047 |
0.011 |
0.015 |
0.057 |
| MeOH:H2O:CAN (60:30:10) |
0.01 |
0.017 |
0.019 |
0.021 |
| pH of mobile phase |
2.63 |
0.745 |
0.042 |
0.054 |
0.145 |
| 2.67 |
0.014 |
0.019 |
0.014 |
0.021 |
| Flow rate (mL min-1) |
0.9 |
0.241 |
0.032 |
0.061 |
0.01 |
| 1.1 |
0.417 |
0.421 |
0.019 |
0.005 |
| Column temperature (°C) |
22 |
0.528 |
0.247 |
0.013 |
0.007 |
| 30 |
0.015 |
0.087 |
0.041 |
0.018 |
| Detection wavelength (nm) |
238 |
0.065 |
0.024 |
0.081 |
0.045 |
| 242 |
0.021 |
0.004 |
0.014 |
0.085 |
|