Parameter Flucloxacillin
Concentration range 25.35 – 152.10 μg/mL
Number 4
Average values 0.0066
Correlation coefficient (R2) 0.995
Relative standard deviation (%) 0.9262
Calibration equation y=156.94x + 0.0699
Limit of Detection 1.2837 × 10-4 μg/mL
Limit of Quantification  3.89 × 10-4 μg/mL
System suitability 0.00253
Method precision 0.25%
LOD = Limit of detection, LOQ = Limit of quantification
LOD=3.3 × σ/S, where σ= SDEV of the responses, S= slope of the regression line
LOQ=10 × σ/S, where σ= SDEV of the responses, S= slope of the regression line
Table 8: Statistical validation of the calibration data for quantitative determination of flucloxacillin.