Substrate Free GHTHase PEG-GHTHase
Km (mM) Vmax
Umg-1 min-1
Kcat
min-1
Kcat/Km
mM-1 min-1
Km(mM) Vmax
Umg-1 min-1
Kcat
min-1
Kcat/ Km
mM-1 min-1
Glutathione 14.3 ± 2.0 277.7 ± 65 0.078 0.0063 20.1 ± 4.0 212.7 ± 57 0.06 0.0029
L-Cysteine 15.1 ± 2.3 200.1 ± 43 0.056 0.0004 17.2 ± 3.2 136.9 ± 29 0.038 0.0023
L-Lysine 40.2 ± 4.2 120.0 ± 25 0.043 0.0012 13.2 ± 2.9 133.3 ± 30 0.037 0.0029
Table 1: Kinetics of substrates specificity for free and PEG-GHTHase towards glutathione, L-cysteine and L-lysine as hydrogen donors acceptor. Means and SE for three replicates were calculated using student t-test.