Amino acid Negative control
(µM)
Free-GHTHase
(µM)
PEG-GHTHase
(µM)
Aspartic acid 181.36 177.6 147.04
Threonine 85.40 68.96 75.12
Serine 146.17 290.32 716.4
Glutamic acid 647.17 936.96 743.48
Glycine 108.68 398.48 155.96
Alanine 123.08 156.08 140.0
Valine 132.53 132.96 104.8
Methionine 330.68 25.9 47.6
Cysteine 267.0 57.9 89.0
Isoleucine 129.06 165.8 149.6
Leucine 266.86 261.36 865.04
Tyrosine 500.0 559.04 508.88
Phenylalanine 45.58 84.8 107.76
Histidine 120.38 110.0 138.4
Lysine 212.48 105.2 122.88
Arginine 266.03 116.08 125.0
Proline 378.935 348.5687 369.9019
Table 4: Serum amino acid concentration of rabbits in response to injection of free and PEG-GHTHase after 6 h compared to negative control.