Figure 2: Long term EGF causes compact MCA formation and increased cell spreading. A) Representative western blot of N-cadherin and vimentin with GAPDH as a loading control over weeks of 10 nM EGF treatment.B) Representative images of MCAs formed from passage controls and EGF treated cells over weeks of 10 nM EGF treatment. Scale bar=100 μmC) Representative 10X images of MCAs and MCA spreading on collage from control, EGF and removal cells. Scale bar=200 μm. D) Data showing area of the formed MCA was significantly, and irreversibly, decreased in EGF treated and removal cells compared to controls, n=6, ***p<0.001. E) Data of area of the spreading of MCAs on collagen showed a significant increase in EGF treated and removal cells compared to controls, n=6, ***p<0.001, *p<0.05. Removal cell spreading was also significantly decreased compared to EGF, #p<0.05.