Figure 4: SiRNA knockdown of N-cadherin in EGF treated cells and SKOV3 cells. A) Representative western blot showing N-cadherin expression in OVCA 433 passage control cells and EGF treated cells (untreated with siRNA) or treated with scramble siRNA or Ncad siRNA, GAPDH shown as a loading control. B) Normalized N-cadherin optical density in passage control and EGF treated cells without siRNA (untreated) or treated with scramble siRNA or Ncad siRNA, n=5. The significant increase in N-cadherin in untreated and scramble (EGF treated cells) compared to passage control cells, ***p<0.001 is blunted by treatment with Ncad siRNA, ###p<0.001 (significant decrease from untreated and scramble). C) Representative western blot showing N-cadherin expression in SK0V3 cells (untreated) andtreated with scramble siRNA or Ncad siRNA, GAPDH shown as a loading control. D) Normalized N-cadherin optical density in SKOV3 cells untreated and treated with scramble siRNA or Ncad siRNA, n=3. Significant decrease in Ncad siRNA treated SKOV3s compared to untreated cells, **p<0.01.