Figure 5: MCA formation and spreading after knockdown of N-cadherin in EGF treated cells. A) Representative images of passage control and EGF treated cells with scramble or Ncad siRNA. MCA formation images taken with 10X objective (scale bar=100 μm) and 20X objective (scale bar=50 μm). MCA spreading images taken with 4X objective (scale bar=200 μm) and 10X objective (scale bar = 100 μm). B) Data showing area of the formed MCA was significantly decreased in EGF (scramble) cells compared to controls, n=6, ***p<0.001. Knockdown of N-cadherin (Ncad siRNA) exhibited a significantly decreased MCA formation area from passage control cells, ***p<0.001 but a significant increase in MCA formation area compared to EGF (scramble) cells, ###p<0.001. C) Data of area of the spreading of MCAs on collagen showed a significant increase in EGF (scramble) cells compared to controls, n=6, ***p<0.001. Knockdown of N-cadherin (Ncad siRNA) resulted in a significantly increased area of MCA spreading compared control cells, *p<0.05 but a significant decrease in MCA spreading area compared to EGF (scramble) cells, ##p<0.01. D) Representative images SKOV3 MCAs untreated, scramble siRNA and Ncad siRNA treated taken with 10X objective, scale bar=100 μm. E) Data showing area of the formed SKOV3 MCAs, n=4. Knockdown of N-cadherin (Ncad siRNA) exhibited a significantly increased area of MCA formation from untreated SKOV3 MCAs, *p<0.05.