Figure 3: ROS changes in HCP mucosa. HCP pouches were excised from (A) sentinel control hamsters; (B) D003 control; (C) sentinel animal cheek pouches incubated with pyocyanin (an ROS inducer); (D) DMBA treatment for 4 wks followed by 2% methylcellulose for 24 h; (E) DMBA treatment for 4 wks followed by 2% D003 extract for 24 h;. The mucosal samples were incubated in MEM containing 15 µM DHR for 30 min and imaged with two-photon fluorescence microscopy at various depths. Photos taken at the top levels of all the tissues were shown as representative samples. Green signal indicates ROS and bars equal 50 µm.