β-emitter Relative Signal
strength
Half-life Signal Containment Biological analyses Signal
amplification
Film autoradiography
3H 1 12.4 years Bench work & monitoring Organic molecules. PTMs e.g.
alkylation, methylation
Fluorometry Days-weeks
32P 90 14.3 days User shielding behind 1 cm of perspex Phosphate transfer. PTM as phosphorylation Intensifying screens Hours-days
Table 2: Comparison of the physical properties and biological application of 3H and 32P.