Figure 4: Western immunoblot analysis of proteins in mycobacterial sonicates with sera of rabbits immunized with pure recombinant PE35 (a), PPE68 (b), ESXA (c), ESXB (d), and ESXV (e). M: Prestained low molecular weight marker. Lane 1: Mycobacterial sonicate from the species indicated. The arrows indicate the protein band reactive with antibodies.