Figure 1: Calcified granulomatous lesions contained granules reacted by anti- LAM mAb in necrotic areas.
(A) Western blot analysis with novel mAb using purified LAM (left lane). Negative control with the primary antibody omitted (right lane). (B) Representative picture of a lesion in the ZN(+) group. (C) Area pointed to by the arrow in (B) with ZN staining. Inset shows acid-fast bacilli in higher magnification. (D) IHC with anti- LAM mAb in the area adjacent to (C). (E) Representative picture of a lesion from the calcification(+) group. (F) IHC with anti-LAM mAb in the area pointed to by the arrow in (E). Granular-positive reaction products were detected. (G) High magnification of the area indicated by the dashed square in (F). (H) IHC with anti-LAM mAb in a ZN(+) granuloma. A few granular positive reaction products were observed within the epithelioid cell layer (arrow). (I) Immunoelectron microscopy with anti-LAM mAb in a lesion from the calcification(+) group. DAB deposition (arrow) was observed around the bacillus-like structure (arrowhead). Scale bar= 500μm in (B)(E), 10 μm in (C)(D)(G)(H), 50 μm in (F), 1 μm in (I).