Figure 2: Calcifications were observed in the lesions positive by real-time PCR for M. tuberculosis and negative by ZN staining. Seventy granulomatous lesions were screened by real-time PCR for M. tuberculosis. Real-time PCR-positive lesions were then checked for acid-fast bacilli by ZN staining and for the presence of calcification in necrotic areas. The lesions were classified into three groups: ZN(+), Calcification (Cal)(-) (Cal(-)), Cal(+), and PCR(-) groups.