Figure 2: Specificity assessment by comparing IF assay and Western blotting results for the detection of MUL2232. NM 20/02 wild type M. ulcerans was compared to a mutant strain overexpressing HspR_2, a transcriptional repressor of MUL2232, resulting in a functional knockdown of MUL2232. The mutant strain was generated from NM20/02 using a HspR_2 harboring plasmid previously described [18]. GroEL served as a loading control. All micrographs were acquired using the same exposure time (950 ms) and detector gain (=1). Scale bar: 2μm