1 2 3 4 5 6 7 1 ITS-1A M. kumamotonense 100%             2 ITS-1B M. kumamotonense 92% 100%           3 ITS M. aurupense 88.40% 77.40% 100%         4 ITS M. terrae (Genotype I) 79% 71.30% 77.20% 100%       5 ITS-1A M. celatum 77.90% 75.60% 72.70% 68.50% 100%     6 ITS-1B M. celatum 76.50% 71.30% 73.10% 65.50% 99.60% 100%   7 ITS M. tuberculosis H37Rv 67.80% 72.30% 69.50% 66.80% 69.40% 66.10% 100%

Table 2: Percentage of sequence similarity of the ITS-1 genomic region. Percentages of similarity between slow grower mycobacteria members of the M. terrae complex, including M. kumamotonense. Percentage of similarity with M. tuberculosis is also showed. The sequences of the two copies of M. celatum and M. kumamotonense are included (labelled as “A” and “B” respectively).Accession number of the sequences of slow grower mycobacteria used for comparison: M. kumamotonense, this work; M. aurupense, DQ523527; M. terrae (genotype I, 11), AJ314868; M. celatum, EF613281 (ITS-1A) and EF613282 (ITS1B); M. tuberculosis, NC000962.