| Gene fragment |
Primers Sequence(5'-3') |
Amplicon size (bp) |
PCR rogram |
| MT-10.3(1M-40S) |
BamHI |
F 5'CGCGGATCCATGTCGCAGATTATGTAC3' |
120 |
94°C,5'
94°C,1'
60°C,1' 25x
72°C,1'
72°C,5' |
| |
SacI |
R 5'TATGAGCTCGGACAGCACGGCCTGCTC3' |
|
| CE15(173G-187A) |
SacI |
F 5'ACGGAGCTCGGGGTGATTTTCTTCTTC3' |
42 |
| |
SalI |
R 5'TATGTCGACTGCTTCGGGCAGCAACTC3' |
|
| MT-10.3(41S-96) |
SalI |
F 5'ATAGTCGACAGTGCTTGGCAGGGTGAT3' |
171 |
| |
HindIII |
R 5'ACAAAGCTTTAGCCGCCCCACTTGGC3' |
|
| MPT-64(91L-205A) |
SacI |
F 5'TATGAGCTCCTCAAGGTCTACCAGAAC3' |
348 |
| |
SalI |
R 5'TATGTCGACGGCCAGCATCGAGTCGAT3' |
|
