Antigens IgA IgG IgM MT10.3 (µg/mL) 1,0 1,0 0,5  Serum (diluition) 1:50 1:400 1:400 Secundary antibody 1:2.000 1:1.500 1:500 MPT64 (µg/mL) 0,5 1,0 1,0  Serum (diluition) 1:50 1:400 1:400 Secundary antibody 1:2.000 1:2.500 1:500 F1 (µg/mL) 0,5 0,5 ND  Serum (diluition) 1:100 1:200 - Secundary antibody 1:2.500 1:10.000 - F2 (µg/mL) 0,5 1,5 1,5  Serum (diluition) 1:50 1:400 1:25 Secundary antibody 1:20.000 1:5.000 1:2.500

Table 2: Conditions used in the ELISA IgA, IgG and IgM to test the new proteins F1 and F2, as well MPT-64 and MT10.3 full proteins. ND: no done.