Figure 1: Changes in pH of the incubation medium of mitochondria in the control and in the presence of GuHCl at different concentrations. 1) Control– sucrose 0.4 M; 2) sucrose 0.35 M and 0.025 M GuHCl; 3) 0.025 M GuHCl;4) 0.1 M GuHCl; 5) 0.2 M GuHCl; 6) 0.3 M GuHCl. The incubation medium contained 18 mM KH2PO4, 0,1% BSA and 1mM glutamate, pH 7,2. Arrows indicate addition of 1mg of mitochondrial protein, 2.75 mg of sodium succinate,and 0.24 mg of sodium ADP. Final volume was 1 ml.