Figure 2: Kinetic curves of changes in mitochondrial volume in the control and in the presence of GuHCl at different concentrations. 1) Control – sucrose 0.4M; 2) sucrose 0.35 M and 0.025 M GuHCl; 3) 0.025 M GuHCl; 4) 0.1 M GuHCl;5) 0.2 M GuHCl; 6) 0.3 M GuHCl. The incubation medium contained 18 mM KH2PO4, 0,1% BSA and 1mM glutamate, pH 7,2. Arrows indicate addition of 1mg of mitochondrial protein, 2.75 mg of sodium succinate, and 0.24 mg of sodium ADP. Final volume was 3 ml.