| Antibodies |
clone |
class |
code |
Antigen unmasking methods: Microwave retrieval |
Dilution |
| Monoclonal Mouse Anti-Human Muscle Actin (SMA) |
1A4 |
IG2a, kappa |
M0851 |
15 minutes heating-induced epitope retrieval (750 W) in 10 mmol/Lcitrate buffer, pH 6 |
1:75 in 0.02 M PBS, pH 7.2-7.6 |
| Monoclonal Mouse Anti-Human Cytokeratin AE1/AE2 |
AE1 and AE3 |
AE1: IgG1, kappa; AE3: IgG1, kappa |
M3515 |
15 minutes heating – induced epitope retrieval (750 W) in 10 mmol/Lcitrate buffer, pH 6 |
1:50 in 0.02 M PBS, pH 7.2-7.6 |
| Polyclonal rabbit Anti-S 100 |
- |
- |
Z0311 Ig fraction |
15 minutes heating – induced epitope retrieval (750 W) in 10 mmol/Lcitrate buffer, pH 6 |
1: 300 in 0.02 M PBS, pH 7.2–7.6 |
| Polyclonal Rabbit Anti-Human Carcinoembryonic Antigen (CEA) |
- |
- |
A0115 Ig fraction |
15 minutes heating – induced epitope retrieval (750 W) in 10 mmol/Lcitrate buffer, pH 6 |
1:100 in 0.02 M PBS, pH 7.2-7.6 |
| Monoclonal Mouse Anti-Human Vimentin (VIM) |
V9 |
IgG1, kappa |
M0725 Culture supernatant |
15 minutes heating – induced epitope retrieval (750 W) in 10 mmol/Lcitrate buffer, pH 6 |
1:30 in 0.02 M PBS, pH 7.2-7.6 |
| Polyclonal Rabbit Anti-Glial Fibrillary Acidic protein (GFAP) |
- |
- |
IS524 |
15 minutes heating – induced epitope retrieval (750 W) in 10 mmol/Lcitrate buffer, pH 6 |
1:30 in 0.02 M PBS, pH 7.2-7.6 |
