Study, Year HPV-DNA detection and typing method
Europe  
St Guily, 2011 [32] SPF10*, INNO-LiPA
Hannisdal, 2010 [33] GP5+/GP6+ touchdown PCR, sequencing
Näsman, 2009 [3] GP5+/6+ and CPI/IIG followed by sequencing, negative samples analyzed using HPV16 type-specific primers
Hammarstedt, 2006 [34] GP5+/6+, CPI/IIG, negative samples analyzed using HPV 16 type-specific primers, samples positive by GP5+/6+ or CPI/IIG were sequenced for type
Mammas, 2006 [46] GP5+/6+, then type-specific PCR for 16/18/33/11
Chen, 2005 [47] Nested PCR with MY09/11 & GP5+/6+, genotype by sequencing
Mellin, 2002 [40] GP5+/6+, negative samples were run with CPI/IIG, positive samples sequenced for genotype
Klussmann, 2001 [36] Nested PCR with A10/A5-A6/A8, and CP62/70-CP65/69a, HPV16 type-specific PCR, typing by sequencing
Mellin, 2000  [35] GP5+/GP6+, positive samples re-run with HPV16/33 type-specific primers
Andl, 1998 [37] Type-specific primers for 6/11/16/18 followed by SB, negative samples rerun using consensus primers, products sequenced for genotyping
Snijders, 1992 [38] GP5+/GP6+ followed by sequencing, type-specific PCR for HPV 16 and 33
Niedobitek, 1990 [39] ISH with probes for HPV16, and HPV 6 and 11
North America  
Ernster, 2009 [48] Type-specific primers for HPV16 and 18
Luginbuhl, 2009 [41] ISH with HPV III family 16B probe (probe cocktail for detection of high risk HPVs)
Pintos, 2008 [14] PGMY09/11, line blot assay for 27 HPV types
Ernster, 2007 [42] Type-specific primers for HPV16 and 18
Sisk, 2006 [49] PGMY09/11, Roche Linear Array for genotyping
Strome, 2002 [43] MY09/11 followed by nested PCR for HPV-negative samples using primers 6582-23D and 7033-22 U, genotyping by sequencing; samples also run with HPV 16 type-specific primers, followed by SB for HPV16-negative samples
Ringström, 2002 [44] MY09/11, typing by restriction enzyme digestion to detect HPV16 and 18
Paz, 1997 [45] MY09/11 and IU/IWDO primers, samples positive by either primer set were analyzed using type-specific primers for HPV6, 16, and 18, samples also analyzed by SB
Asia  
Chien, 2008 [18] MY09/11, HPV positive samples analyzed by ISH for HPV6, 11, 16, 18, samples negative by ISH analyzed by linear array genotyping test (Roche)
Li, 2003 [19] GP5+/6+, CP65/70ct-CP66/69ct, FAP59/64, A5/A10-A6/A8, type 16-specific primers, sequencing for type
Oceania  
Li, 2003 [1] GP5+/6+, CP65/70ct-CP66/69ct, A5/A10-A6/A8, type 16-specific primers, sequencing for type
Table 3: HPV DNA detection and genotyping methods used in the studies presented in Table 1 and 2. (SB=Southern Blotting, ISH=In-situ hybridization).