Figure 3:Pattern of Fos expression in DCN. (A)The dashed lines in ipsilateral DCN (DCNi) mark two different ROIs: fusiform layer and deep layer, for statistical analysis of positively stained large nuclei (30-100 μm2). Scale bar: 200 μm. Inset: Contralateral DCN (DCNc) shows little Fos expression. (B) The bordered area marks the ROI for statistical analysis of positively stained small nuclei (8-29 μm²), called vertical cell layer. The inset shows large and small nuclei prior to (upper part: large vs. small black dots) and after (lower part: white large nuclei; greyish small nuclei) identification by image analysis. Scale bar for inset: 20 μm. Shown are the patterns of a nimodipine-treated rat. (C-E) Fos expression following different types of stimulation within the non-treated group. Shown is the quantification of positively stained large nuclei in the fusiform layer (C) or deep layer (D) of DCN, as well as stained small nuclei within the vertical cell layer (E). (F-H) Fos expression after different types of stimulation of nimodipine-treated animals. Analogue to (C-E) it is shown the quantification of positively stained large nuclei in the fusiform layer (F) or deep layer (G) of DCN and the small Fos positive nuclei in the vertical cell layer of DCN (H). For significant differences against control (a) see text. (***): p<0.001; (**): p<0.01; (*): p<0.05; Co: Control group; Impl: dummy implantation of electrode carrier; i: ipsilateral; c: contralateral.