| Parameters | Observation | 
      
        | Linearity    range ( ng per band) | 300    - 1800 | 
      
        | Limit of    detection (ng) | 17.21 | 
      
        | Limit of    quantitation (ng) | 52.18 | 
      
        | % Recovery ( n =  9) | 100.50 | 
      
        | % RSD | 0.37 | 
      
        | Precision (% RSD) |  | 
      
        | Repeatability    ( n = 6) (standard) | 0.88 | 
      
        | Intra- day (n    = 6) )(standard) | 0.06 | 
      
        | Inter-day (n =    6) )(standard) | 0.37 | 
      
        | Ruggedness  (%RSD) |  | 
      
        | Analyst –I (n    = 6) )(standard) | 0.76 | 
      
        | Analyst –II (n    = 6) (standard) | 0.75 | 
      
        | Robustness | Robust | 
      
        | Specificity | Specific |