| 
      
        | Parameters | 2D method | 2DDmethod |  
        | Range(μg/ml) | 2-12 μg/ml | 2-14 μg/ml |  
        | Slop | 0.0465 | 0.0275 |  
        | S.E. of slop | 0.0004 | 0.0003 |  
        | Intercept | -0.0255 | 0.0083 |  
        | S.E. of Intercept | 0.0032 | 0.0034 |  
        | r | 0.9998 | 0.9994 |  
        | Mean±RSD% (accuracy) | 100.36±1.836 | 99.81±1.889 |  
        | LOD | 0.2182 | 0.4511 |  
        | LOQ | 0.6612 | 1.3672 |  
        | Repeatability % | 1.471 | 1.863 |  
        | Intermediate presicione % | 1.616 | 1.868 |  |