| TYPE |
SAMPLE POLARITY |
MOLECULAR WEIGHT RANGE |
STATIONARY PHASE |
MOBILE PHASE |
| Adsorption |
non-polar to somewhat polar |
100 - 104 |
silica or alumina |
non-polar to polar |
| Partition (reversed-phase) |
non-polar to somewhat polar |
100 - 104 |
non-polar liquid adsorbed or chemically bonded to the packing material |
relatively polar |
| Partition (normal-phase) |
somewhat polar to highly polar |
100 - 104 |
highly polar liquid adsorbed or chemically bonded to the packing material |
relatively non-polar |
| Ion Exchange |
highly polar to ionic |
100 - 104 |
ion-exchange resins made of insoluble, high-molecular weight solids functionalized typically with sulfonic acid (cationic exchange) or amine (anionic exchange) groups |
aqueous buffers with added organic solvents to moderate solvent strength |
| Size-Exclusion |
non-polar to ionic |
103 – 106 |
small, porous, silica or polymeric particles |
polar to non-polar |
