Parameter TLC-densitometric method HPLC method Range MTR DIQ MTR DIQ 1-20 µg spot-1 0.5-10 µg spot-1 0.01-1 mg mL-1 0.005-0.5 mg mL-1 Slope 0.079 0.438 39.69 38.16 Intercept 0.092 0.738 - 0.486 0.011 Mean 99.87 100.15 99.92 100.15 ± S.D. 0.365 0.593 0.433 0.483 Variance 0.133 0.352 0.187 0.233 Coefficient of variation 0.365 0.592 0.433 0.482 Correlation coefficient (r) 0.9998 0.9999 0.9999 0.9999 R.S.D. (%) 0.365 0.592 0.433 0.482

Table 1: Assay parameters and validation sheet for determination of metronidazole (MTR) and diiodohydroxyquine (DIQ).