Northeast Agricultural University, China
Title: A bispecific antibody against IL-1 and IL-17A is beneficial for rheumatoid arthritis
Guiping Ren has obtained excellent education and research trainings from Northeast Agricultural University in China. She received her Bachelor, Master and PhD degrees from Northeast Agricultural University. She has been employed as associate professor of Northeast Agricultural University since 2005. She has been appointed as department head of Department of Microbiology and Biochemical Engineering, College of Life Science, Northeast Agricultural University since 2007. Her research focused on rabies virus for one year as visiting scholar in The University of Georgia, USA. She has been active in the field of Pharmaceutical Biotechnology and Molecular Bioglogy.
IL-1β is the pivotal cytokine and plays an important role in rheumatoid arthritis (RA). More recently, the biological therapy targeting this cytokine has been impressively effective for many RA patients, however, it remains insufﬁcient in some patients. One of the reasons for these futures may be due to multiple cytokines involved in the disease process. In the present study, a single-chain bispecific antibody (scBsAb1/17) was constructed against both human IL-1and human IL-17A which is mediator for several key cytokines involved in RA process such as TNF-ɑ and IL-6. A number of in vitro assays demonstrated that scBsAb1/17 simultaneously bound to both targets with similar antigen-binding affinity as individual single-chain antibody molecule (anti-IL-1 scFv or anti-IL-17A scFv). Mice with collegen-induced arthritis (CIA) were administrated with either scBsAb1/17 or individual single chain antibody alone, and noticed that treatment with scBsAb1/17 significantly ameliorated clinical signs and alleviated histological lesion of CIA mice compared to treatments with anti-IL-1 scFv or anti-IL-17A scFv alone. Production of CII-specific antibodies in scBsAb1/17 treated CIA mice was substantially lower than that of single-chain antibody-treated CIA mice. In addition, scBsAb1/17 was more potent in inhibition of collagen-specific proliferation of splenocytes and mRNA expression of TNF-ɑ, IL-6, IL-2, IL-1 and IFN-γ in the spleen of CIA mice compared to single-chain antibody alone. These results suggest that scBsAb1/17 appears more beneﬁcial in rheumatoid arthritis than monovalent single-chain antibody molecules.
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