Southern Connecticut State University, USA
Title: A new preclinical human cancer model demonstrates successful growth of solid tumors of diverse tissue origins in unfertilized avian eggs
Sarah Crawford is a Professor in Southern Connecticut State University, USA. Her research interest includes genetics and cancer biology.
A novel pre-clinical approach developed in this laboratory for the fi rst time demonstrates the successful growth of human solid tumors of the brain, breast, colon and pancreas in unfertilized chick eggs. Th e research suggests that the avian system is a suitable and in many ways more advantageous culture system for the growth of human tumors than current in vitro and mouse model systems. Growth advantages off ered by the avian system include: • High success transfer rate (at least 80%) from in vitro spheroid culture to avian culture. • Rapid establishment within days of solid tumor growth. • Broad spectrum application to tumors of diverse tumor type grown successfully in this system, including tumor spheroids of the brain (glioblastoma), pancreas (ductal adenocarcinoma), colon, and breast (primary and metastatic origins). • Capacity for long-term cultivation not possible in fertilized chick eggs by serial transfer. • Avian embryonic environment aff ords biochemical, biophysical parameters and determinants of growth properties diffi cult to achieve in adult animal models or in vitro systems. • Ease of culture manipulation and tumor growth assessment. • Tumor growth in avian eggs displays heterogeneous growth parameters more similar to in vivo growth than spheroid cultures, including clear distinctions between necrotic tumor centers and active growth zones, increased invasiveness compared to traditional spheroid growth models and histological heterogeneity similar to that observed in human tumor specimens observed surgically and histologically. In summary, research demonstrating the simplicity, effi cacy and greater similarity in tumor growth properties in the avian system as compared to in vitro spheroid culture methods will be presented, including data showing the use of this model system to assess the potential effi cacy of novel therapeutic approaches and as a comparative tool for assessing pre-clinical versus patient treatment responses.