Dalian Medical University, China
Title: Alpha-lipoic acid produces bone-protective effects via modulating Nox4/ROS/NF-κB and Wnt/Lrp5/β-catenin signaling pathways in H2O2-treated MC3T3-E1 cells and ovariectomy rats
Huijun Sun is the Professor of Pharmacology of Dalian Medical University, China. Her major is pharmacology of metabolic diseases, such as hyperlipidemia, atherosclerosis and osteoporosis and she has published more than 50 papers in reputed journals. She completed her Ph.D in Dalian Medical University, China and have ever studied and worked in the University of Tsukuba, University of Yamanashi of Japan.
Recently, increasing studies indicated the relationship between oxidative stress and osteoporosis. And some studies have been done to detect the possible beneficial effects of Alpha-lipoic acid (ALA), a potent antioxidant, on osteoporosis in vivo and in vitro. However, the detailed mechanism(s) underlying the bone-protective action of ALA are still poorly understood. The present study aims to examine the mechanisms by which ALA produces bone-protective effects in vitro and in vivo on base of its antioxidant effects, thus the effects of ALA on H2O2-treated MC3T3-E1 pre-osteoblasts and ovariectomized osteoporosis rat model were investigated by using bone biomechanical testing, microcomputed tomography, western blotting and qRT-PCR analysis. The results showed that ALA promoted osteoblastic formation, inhibited osteoblastic apoptosis, increased OPG/RANKL ratio and enhanced bone formation in vitro and prevented bone loss in vivo. And the effects of ALA were via modulating Nox4/ROS/NF-κB and Wnt/Lrp5/β-catenin signaling pathways, which revealed possible mechanisms of bone-protective effects of ALA. The current study indicated that ALA might be a candidate for the prevention and therapy of osteoporosis clinically at the end of advanced studies.