Catholic University of Pernambuco
Title: Biotechnological valorisation of raw glycerol from biodiesel to production biomass and single cell oil by Mucor circinelloides UCP 050
Carlos Alberto Alves da Silva is PhD in Biotechnology by the Technical University of Lisbon - Instituto Superior Técnico (1998) and Post Doctorate at the Institute of Chemistry Sarria - Ramon Llull University, Barcelona (2007-2008). He is currently Associate Professor III and teaches courses in the Environmental and Chemical Engineering, Chemistry Degree and Effective Researcher at the Nucleus for Research in Environmental Science and Biotechnology (NPCIAMB, Catholic University of Pernambuco).The main research activities in Biotechnology, Industrial and Environmental Microbiology are: Bioprocess of Separation, Isolation/Identification of Microorganisms, Production and purification of microbial enzymes.
This study concerns to the evaluation of glycerol waste (GW) from biodiesel as carbon source for biomass, single cell oil and fatty acid production by a oleaginous fungi Mucor circinelloides isolated from mangrove land (Pernambuco, Brazil). Studies were also carried out to determine the effects of GW on superoxide dismutase, catalase and gluthatione S-transferase. The use of sudan black staining revealed lipid inclusion bodies and an increase in the mycelial branching related to glycerol waste (GW) presence and concentration. Growth on 8% glycerol waste rendered a biomass production of 11.83g/L, compared to 5.62 g/L and 5.515 g/L using glucose and commercial glycerol (CG), respectively. A single cell oil content of 52.91% was obtained at 8% of glycerol waste, and showed ω-3Linolenic acid (C18:3) (45.4%) as the main fatty acid from biomass. Activities of 67U/mg protein, 65U/mg protein and 0.744U/mg protein were obtained for superoxide dismutase, catalase and gluthatione S-transferase, respectively, in 8% of the residue. These data pointed out the raw material exploitation and efficiency as an alternative carbon substrate to glucose for bioprocesses related to biomass, single cell oil, fatty acids and oxidative enzymes production. These data also are of great importance once is based on the use of microbial diversity from mangrove land.