Cairo University, Egypt
Title: Cardiac proteome alterations induced by diabetic oxidative stress on in OLETF rats
Abdelbary Prince is a Biochemist and Molecular Biologist. Over the past 10 years, he has served at Faculty of Veterinary Medicine, Cairo University. He received his Doctorate in Biochemistry and Molecular Biology. Dr. Prince did his Master's and Doctorate research in the laboratory of Dr. Han at Inje University, South Korea and his post-doctoral work as Mass Spec Specialist in the laboratory of Cregan at USDA. While teaching 15 contact hours a semester, advising students and being involved throughout Cairo University campus, he has maintained a successful research. For the past 5 years, Abdelbary was worked in the Laboratory of Molecular Epidemiology to focus on genotyping of Avian Influenza isolated from Egyptian farms. He has participated in organization of many workshops and conferences in the field of Genomics and Proteomics as lecturer and organizer.
Diabetic cardiomyopathy has been documented as an underlying etiology of heart failure among diabetics. Although oxidative stress has been proposed to contribute to diabetic cardiomyopathy, much of the evidence lacks specificity. Furthermore, whether alterations occur at the cardiac proteome level in diabetic cardiac complications with attendant oxidative stress remains unknown. Therefore, we sought to identify cardiac protein changes in relation to myocardial oxidative stress that are specific to diabetic cardiomyopathy. Animal model of type 2 diabetes (OLETF rat) was used to investigate the alteration of myocardial proteome in diabetic cardiomyopathy. OLETF rats were examined for diabetic cardiomyopathy at 35 weeks of age by histopathogical and histochemical analyses. Myocardial oxidative stress was shown in diabetic rats, as indexed by significant increase in mitochondrial superoxide formation. In-depth mining of the diabetic myocardial proteome by proteomic analysis utilizing two-dimensional gel electrophoresis and mass spectrometry (2DE/MS) techniques revealed down-regulation of antioxidant and anti-apoptotic proteins and up-regulation of fatty acid oxidation related proteins in diabetic hearts. These results characterize a role of substrate switch to fatty acid utilization in alteration of metabolic pathways in diabetic cardiomyopathy.