Beni-Suef University, Egypt
Title: Circulating microRNAs (miR-21, miR-223, miR-885-5p) along the clinical spectrum of HCV-related chronic liver disease in Egyptian patients
Mona Zaky Nasser has obtained her Master and MD degree from Cairo University. She has spent one year (2008-2009) as a Postdoctoral Researcher in Washington University in ST Louis, USA. She has been involved in the establishment of the molecular diagnostic unit in Beni-Suef University. Moreover, she is the Director of the quality assurance program in the clinical chemistry unit in Misr University for Science and Technology. She has supervised many Post-graduate candidates and participated in number of conferences where she has presented few posters and oral presentations. Her research interest includes molecular diagnostics and genetic background of human cancer.
Introduction/Aim: Increasing evidence suggests thatseveral pathological hepatic diseases have been related to alterations of miRNAs expression.Th e present study was designed to assess the signifi cance of serum miR-21, miR-223, and miR-885-5p as potential biomarkers in diff erent clinico-pathological sequalae of HCV-related chronic liver disease. Patients and Methods: Serum miR-21, miR-223, and miR-885-5p were quantifi ed by real-time quantitative PCR in 60 Egyptian patients with HCV-related liver disease in addition to 25 healthy controls. HCV patients were classifi ed into: chronic HCV (n=15), liver cirrhosis (n=15), and hepatocellular carcinoma (HCC) (n=30). Results: Serum levels of miR-885-5p in cirrhotic patients (with or without HCC) were signifi cantly higher than the noncirrhotic patients; p=0.007 and healthy control; p=0.001. However, no such signifi cance was detected between HCV patients with and without HCC; p=0.12. Serum miRNA-885-5p was able to discriminate cirrhosis ± HCC from healthy controls using ROC analysis: AUC 0.85, 87% sensitivity and 80% specifi city. As regards serum miR-21, HCC patients had signifi cantly higher levels than non-HCC patients (non-cirrhotic and cirrhotic groups); p=0.048 and the control group; p=0.002. ROC could diff erentiate HCC from control group; AUC 0.89, 80% sensitivity, 80% specifi city. Serum albumin and bilirubin were signifi cantly correlated with miRNA-885-5p(r=-0.27, p=0.04) (r= 0.42, p=0.001) respectively, but such correlation was not observed with serum miRNA-21. In contrast, mi RNA 223 showed no signifi cant diff erence across the studied groups. Conclusion: Along the spectrum of HCV-related chronic liver disease, miR-885-5p could be a potential marker for advanced liver damage while miR-21 could be a helpful diagnostic marker for HCC.