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Salim Mattar

University of Cordoba, Colombia

Title: Epidemiological surveillance of herpesvirus encephalitis in Cordoba, Colombia 2009-2011

Biography

S. Mattar Ph.D, has research experience of 25 years on tropical infectious diseases He actuatlly is the director of Tropical Reserach Institute at University of Cordoba (http://web.www3.unicordoba.edu.co/es/iibt), He has published more than 100 papers in several peers journals. He is full professor of Microbiology and Immunology at University of Cordoba.

Abstract

We conducted a cross-sectional descriptive study of cases compatible with viral encephalitis in three hospitals in the city of Monteria. We included 265 specimens of cerebrospinal fluid (CSF) of adults (n=118) and pediatric (n= 147) patients with clinically suspected of encephalitis. Cytochemical analysis and microbiological tests (Gram stain and culture) were performed; multiplex and nested PCR detection using 16 oligonucleotides for herpes simplex virus 1 and 2 (UL30 DNA polymerase) were done. The primers used were: Epstein Barr virus (gp71 DNA polymerase), Cytomegalovirus (UL54 DNA polymerase) and Varicella zoster virus (DNA polymerase). Herpesvirus DNA was detected in 57 (21.5%) samples, with the following distribution: 47 (17.7%) Herpes simplex virus 1 and 2, 7 (2.6%) Cytomegalovirus, 4 (1.5%) Varicella zoster virus and 2 (0.75%) Epstein Barr virus. Co-infection was seen in 3 patients, VZV+HSV1-2 (n=1) and CMV+HSV1-2 (n=2). A 52.6% (30/57) of adult patients shown viral DNA detection, the distribution was: HSV1-2 (n=22), CMV (n=4), VZV (n=1) and co-infections (VHS1-2+CMV ( n=2) , VHS1-2+VVZ (n=1). In a pediatric population 47.3% (27/57) DNA was identified with HSV1-2 (n=22), VZV (n=2), CMV (n=1), EBV (n=2). Three parameters of cytochemical analysis (glycorrhachia, proteinorhaquia and pleocytosis) were abnormal in 15.7% (9/57) of patients; 21% (12/57) of patients (adults n=11, pediatric n=1 ) were diagnosed with HIV. Patients sequelae were observed in 12.2% (7/57) and mortality was 10.5% (6/57). This is the first surveillance of herpes encephalitis conducted in Cordoba; the findings contribute to the epidemiology of encephalitis and clinical management of patients.

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