Universiti Sains Malaysia, Malaysia
Title: In situ detection of Polyalthia longifolia leaf extract in developing natural anti-cancer therapeutic agent: Optical and electron microscopy evidence
Soundararajan Vijayarathna is a second year Doctoral student in Molecular Medicine at Universiti Sains Malaysia (USM). She has published more than 12 papers, 5 proceedings and contributed to a book chapter. Currently, her fi eld of research addresses the relationship of microRNAs with cancer biology and the role of natural products as anti-cancer agents. She won the most “Excellent Paper” award for her oral presentation at 2012 3rd International conference on Biotechnology and Food Science in Bangkok, Thailand and been nominated for Best Thesis Award by the Institute for Research in Molecular Medicine in USM for her MSc thesis.
Optical and electron microscopes are extensively employed scientifi c techniques, which gauge cytological changes as incur in cell cytotoxicity. Th e microscopic images relinquish topographical, morphological and compositional evidences that infer cell mechanism in response to anticancer agent. On that ground, this study engaged numbers of microscopy methods to detect in situ anticancer activity of Polyalthia longifolia against cervical cancer cell line, HeLa. Th e P. longifolia being the most important indigenous medicinal plants, are found throughout Malaysia and generally use by traditional healers to treat various diseases. Th e MTT assay results disclosed the lowest IC50 value of 14.181 μg/ml as P. longifolia leaf extract debilitate HeLa cells. Th e eff ect of this plant extract on HeLa cells were observed at 0, 6, 12, 24 and 36 hours using Light Microscope (LM), Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM) and HoloMonitor (HM). Th e cytological observations underlined cell shrinkage, nuclear and chromatin condensation, multinucleation, membrane blebbing, punctures, cytoplasmic extrusions and formation of apoptotic bodies, which are correlating within LM, SEM, TEM and HM images. Further biochemical tests were performed to verify this apoptosis resemblance. P. longifolia is deduced to eff ectuate distinctive morphological features of cell death in conformity to apoptosis. Th e fi ndings corroborate P. longifolia as a potential natural therapeutic agent in combating cervical cancer, which ranks the world’s second largest cause of female cancer mortality.