Iran University of Medical Sciences, Iran
Title: In vitro regenerative function of Silymarin in preparation of hematopoietic progenitor cells
Dr Minoo Shahidi is an academic member of the Hematology group at the Iran University of Medical Sciences (IUMS). In 2010, she achieved her PhD from the University of Surrey, UK. During her several year careers as a lecturer at IUMS, she published a number of books and papers. In addition, she conducted some projects and received certificates of the invention. She also dedicates herself in providing help to her postgraduate students as well as to professionals and offers tutorials for them to pass their thesis via her projects in the field of novel treatment approaches for leukaemia and hemostasis.
Statement of the Problem: Since human umbilical vein endothelial cells (HUVEC) can be simply collected, developing of hematopoietic progenitor cells from these cells may improve hematopoietic cell engraftment for irregular cases with limitations for receiving compatible blood components. Regenerative functions of Silymarin, a mixture of polyphenolic flavonoids derived from milk thistle, have been reported previously. Accordingly, there are some reports of Silymarin effect in regeneration and treatment of skin disorders, but no effect of this polyphenolic flavonoid on hematopoietic cells has been reported so far. The purpose of this study was to investigate the direct effect of Silymarin on conversion of HUVEC to hematopoietic cells. The effect of Silymarin on all the main hematopoietic lineage markers in HUVEC was investigated after 24h by flow cytometry and immunocytochemistry. Treatment of endothelial cells with Silymarin after 24h significantly increased the number of the cells expressing hematopoietic progenitor markers. However, no significant expression of the lineage-specific markers was found during 24h. Taken together, our in vitro findings revealed the potential of Silymarin to switch human