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Eman H.Abdel-Rahman

Eman H.Abdel-Rahman

National Research Center, Egypt

Title: Induction of protective cellular and humoral responses against fasciolosis in rabbits using immunoaffinity fraction of Fasciola gigantica excretory secretory products

Biography

Eman Hussien Abdel-Rahman is currently working as Professor in National Research Center, Dokki, Cairo, Egypt since 2005. In 1990, she was appointed as Assistant Researcher at the National Research Center, Dokki, Cairo, Egypt. In 1995, she was appointed as Researcher at the National Research Center, Dokki, Cairo, Egypt. In 2000, she was appointed as Associate Professor at the National Research Center, Dokki, Cairo, Egypt. In 2005, she was appointed as Professor at the National Research Center, Dokki, Cairo, Egypt. Eman Hussien Abdel-Rahman received her B.Sc. in Zoology in 1981 at Cairo University, Egypt. She got M.Sc. in Immunoparasitology in 1990 from Cairo University, Egypt. Dr. Eman Hussien Abdel-Rahman obtained Ph.D. from Cairo University, Egypt in 1995 in Immunoparasitology. Eman Hussien Abdel-Rahman’s current research interests are Immunoparasitology, Biological Control, DNA Technology, Glycoprotein Antigens, Parasitology.

Abstract

Fasciolosis due to Fasciola gigantica or F.hepatica causes significant production losses in animals, as well as being a zoonotic disease of global importance. In an attempt to develop vaccine against fasciolosis in rabbits, an immunoaffinity fraction of F.gigantica excretory secretory products was isolated. The fraction possesses 87.67% of the initial antigenic activities in ES products with 2051.5 fold increase in specific activity compared to crude extract. It consists of two bands of molecular weight 27KDa and 23.5KDa as revealed by SDS-PAGE. Vaccination of rabbits twice with the fraction resulted in 85.7% reduction in worm burden. It is also resulted in high antibody IgG levels as proved by ELISA.Where the highest IgG response was observed at two weeks post first immunization compared to non-vaccinated infected control rabbits. The level of IgG increased at four weeks post infection and remained stable to the end of the experiment. A significant expression of IL-4 and INF-gamma was observed in vaccinated rabbits starting one week until thirteen weeks post infection. The level of IL-4 was significantly higher than the level of INF-gamma throughout the experiment. Collectively, the current results suggest promising immunoprophylactic potentials of the immunoaffinity fraction of ES products of F.gigantica against fasciolosis in rabbits through induction of both cellular and humoral responses.

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