Chinese Academy of Sciences, China
Title: Marine fish genetic resources cryoperservation and application in breeding
Qinghua Liu, associated professor, Institute of Oceanology, Chinese Academy of Sciences. Her reseach areas are: fish reproductive physiology, reproduction and development of marine fish species. She has published more than 40 papers in some reputed journals.
Primordial germ cells (PGCs) are the precursors of gametes responsible for genetic transmission to the next generation. They provide an ideal system for cryopreservation and restoration of biodiversity. Recently, considerable attention has been raised to isolation, cryopreservation and transplantation of PGCs between species. However, the specification and development of PGCs remain elusive in marine economic fish species, impeding the development of PGCs manipulation technology in those species. In order to obtain the basic information of PGCs and put forward the manipulation technology, the development of PGCs was analized in two marine fish species by germ cell specific genes. We cloned vasa and dnd gene in turbot and the red seabream. By whole mount in situ hybridization, we have illustrated the specification and migration of the PGCs in turbot and the red seabream. Besides, we also investigated the function of the red seabream vasa transcriptional regulatory sequences (Pmvas), using medaka as a model system. We proved that the Pmvas was able to label the PGCs in medaka by GFP-Pmvas3’UTR mRNA microinjection and transgenic fish production under control of Pmvas. Furthermore, we isolated and cryopreserved the labeled PGCs and spermatogonia successfully. The results serve for the germ cell manipulation and provide an ideal alternative method for preservation of genetic resource of marine fish.