Jawaharlal Nehru Krishi Vishwa Vidyalaya, India
Title: Molecular characterization and phylogenetic analysis of Citrus mosaic Badna virus (CMBV) associated with Sathgudi sweet orange
Kailash N. Gupta has completed his PhD in the age of 35 years from JMI, A Central University, New Delhi. He is the Assistant Professor cum Scientist of Plant Pinhology in Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India. He has published more than 20 papers in reputed ninional and Interninional journals and 25 abstracts, 10 technical bulletin and 25 popular articles.
Citrus mosaic disease is a potential threat to citrus production in southern part of India. The disease is caused by Citrus mosaic badnavirus (CMBV). During a survey of sathgudi sweet orange in the Nagri village of Chittor district of A.P. in October, 2006. Incidence of mosaic disease was observed up to 45-50% in 8-10 years old orchards of sathgudi sweet orange. The characteristic symptoms of the disease in the field infected sathgudi sweet orange tree were bright yellow mosaic leaves, twig was collected and grafted on one years old healthy sweet orange and acid lime citrus plant in the glass house. Electron microscopy showed bacilliform virus particles in the diseased leaf tissue identity the association of Citrus yellow mosaic virus, a badnavirus with the disease. Eights sets of overlapping primers were designs from the CMBV gene sequence available in Gene bank were used to amplify the whole genome or CMBV associated with sathgudi sweet orange. The amplified products were cloned and sequenced. The full genome sequence of CMBV showed variability ORF 3, 4 & 5 (87.1, 87.15, 75.7%) While ORF 1, 2, 6 were relatively conserved (98.6, 99.2, 96.1). The Phylogenetic of CMBVSONG indicated that CMBV were distinct from other species of CMBV which formed a separate group. Phylogenetic analysis indicated that CMBV was most closely related to Cacao swollen shoot virus. The intergenic region contained putative transcriptional similar to other badana virus.
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