Central Institute of Medicinal and Aromatic Plants (CSIR), India
Title: Purification, characterization and in vitro activity of 2, 4-tert-butylphenol from Pseudomonas putida PsF84: conformational and molecular docking studies
Seema completed her PhD in Biological Science in the year 2014.Her area Of research is in microbial technology, agrotechnology, plant pathology.She has worked on isolation, purification and characterization of bioactive metabolites from Pseudomonas and Streptomyces species and their application in disease management. she is expertise in Plant-microbe interaction. She has established strategies to manage the root-rot wilt disease of pyrethrum (Chrysanthemum cinerariifolium) disease caused by Rhizoctonia solani, Fusarium oxysporum etc. using pathological and computational approaches. Planning for ecofriendly management technologies to manage fungal diseases on medicinal and aromatic plants and phytoremediation assisted technologies. She was a member of the core committee for successfully organizing a two day symposium “JIGYASA-2014” (13th-14th December), organized by CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow
New and novel solutions are sought for improving productivity and sustainability in agriculture. Plant growth-promoting bacteria (PGPB) present an alternative to the use of chemical fertilizers and pesticides in enhancing crop productivity. A novel strain of Pseudomonas putida, PsF84 was isolated from tannery waste soil, Jajmau, Kanpur, India. 16S rRNA gene sequence phylogenetic analysis confirmed the taxonomic affiliation of PsF84 as P. putida. An antifungal volatile compound (VOC) was active against hyphal growth of Fusarium oxysporum (strain CIMAP IMI 357464) in vitro was isolated from strain PsF84 by using chromatographic techniques. The molecular formula of the antifungal VOC was deduced to be C14H22O by EI-MS and 1D and 2D NMR spectral analysis. The antifungal VOC (2, 4-di-tert-butylphenol) was found to be effective against agriculturally important fungi, viz., F. oxysporum in inhibiting spore germination and hyphal growth. Molecular docking analysis of 2,4-tert-butylphenol with β-tubulin further validated the potential of β-tubulin binding in F.oxysporum. The sequence-based prediction of 3D structure of β-tubulin protein of F. oxysporum was also done to analyze the properties and function. To our knowledge, this is the first report of antifungal VOC (2, 4-di-tert-butylphenol) produced by P. putida PsF84 which can be a potent inhibitor of β-tubulin of F. oxysporum.
Keywords: Pseudomonas putida, 2, 4-di-tert-butylphenol, Fusarium oxysporum, Molecular modeling, Docking, β-tubulin.