Hamdard University, India
Title: Recent Applications of Nano-LC-MS in Molecular Toxicology
Shahzad Ahmad has completed his Master of Science in Forensic Science at the age 23 from Osmania University, Hyderabad (India). He served as ‘consultant for forensic science’ at Scope eKnowledge Centre, Pvt Ltd, Chennai, India and pursuing PhD (Toxicology) in the Department of Medical Elementology and Toxicology, Jamia Hamdard, New Delhi, India. He has been awarded Basic Research Fellowship (Senior Research Fellow) from University Grant Commission, Government of India. He has published three research papers in reputed journals and also serving as reviewer for some journals.
Endocrine disrupting chemicals (EDCs) are synthetic compounds used for diverse range of application and interfere with endocrine system through various ways. Accordingly, the endocrine disruption is one the focal area of toxicology. 2D nano liquid chromatography coupled with mass spectrometry (nano-LC-MS) recently attracted the notion of molecular toxicologists due its role in proteomic analysis. Proteomic analysis is the keen interest of molecular toxicologists to understand the toxicity mechanisms of EDCs and other toxicants. Enhanced sensitivity is the main advantage of 2D nano-LC-MS which facilitates the more concentrated bands of peptide in MS offering the determination of low abundant compound in limited sample. Molecular targets of EDCs and other toxicants include various biochemical indices such as proteins or enzymes, therefore the proteomic analysis on exposure to various EDCs helps to find the toxicity mechanisms of these toxicants. In our previous research, we virtually found the interactions of some EDCs with the biochemical indices of glucocorticoid biosynthetic pathway which is the main pathway of adrenal cortex, indicating the potential of EDCs to inhibit the various proteins of adrenal gland. The proteomic analysis of adrenal gland in response to EDCs by 2D nano-LC-MS may reveal the toxicity mechanism of EDCs and helps in risk assessments. Therefore, in the same way 2D nano-LC-MS shows the potential to become the powerful research tool in the field of molecular toxicology.