National Institute on Aging, NIH, USA
Title: Red Blood Cell (RBC) oxidative stress contributes to reduced RBC deformability and oxygen delivery leading to the occurrence of anemia
Joy G. Mohanty completed his Ph.D. in Chemistry in 1977. Following his Ph.D. he has been involved in biomedical research at various institutions like, NIH, Northeast Ohio Universities College of Medicine, University of Montreal, McGill University and Hahnemann University Medical School (presently xel University College of Medicine) prior to joining NIH as a researcher in 2003. Since then, he has been involved in studies on hemoglobin oxidation mechanisms, red blood cell (RBC) deformability changes, RBC properties in mouse model involving oxidative stress, RBC properties in Alzheimer’s disease patients and RBC properties in human subjects having anemia. He has published several papers in the fi eld with more than 40 papers including other research areas in reputed journals and has reviewed several research manuscripts of different journals.
In mammals, since red blood cells (RBCs) are the only cells that carry oxygen to deliver to tissues, these cells are exposed to the highest concentration of oxygen. Work from our laboratory has shown that under hypoxic conditions (such as when RBCs travel through microcapillaries), hemoglobin, the most predominant protein in RBCs, undergoes auto-oxidation producing reactive oxygen species (ROS) like superoxide and hydrogen peroxide. In fact, RBCs have an extensive antioxidant system to eliminate the formation of ROS as soon as they are formed. Even then, an increase in RBC oxidative stress has been reported in many pathological conditions associated with various diseases and aging. Our work has demonstrated that hemoglobin autoxidation in RBCs eventually undergoes further oxidation, perhaps when hemoglobin binds to membranes, thus escaping its antioxidant system in hypoxic conditions and breaks down itself to produce heme degradation products. Th ese heme degradation products are fl uorescent, stable and thus could be easily measured in situ by a spectrofl uorimeter. Our results show that there is an increase in the fl uorescent heme degradation products in diff erent pathological conditions as well as in vitro RBC aging. Hence, the level of heme degradation products in RBCs is a marker of RBC oxidative stress and may be an indicator of RBC health.