Keio University School of Medicine, Japan
Title: Renin-Angiotensin System (RAS) involvement in the oxidative stress-Induced impairment of retinal neuronal cells
Yoko Ozawa M.D., Ph.D. works as a Chief of the Medical Retina Division (Age-related macular disease; AMD division) and also a vitreoretinal surgeon, as well as a Lab Chief of the Laboratory of Retinal Cell Biology (RCB lab). Her main research interest is the neuroprotection and oxidative stress in the retinal diseases, such as AMD and diabetic retinopathy.
Purpose: We previously reported that neurons in the inner layer of the retina are impaired in diabetes through angiotensin II type 1 receptor (AT1R) signaling and oxidative stress. Although induction of oxidative stress in the downstream of AT1R is wellknown, infl uence of oxidative stress on AT1R signaling in the retinal neurons remains to be elucidated. In this study, we analyze the influence of oxidative stress on AT1R signaling in retinal neuronal cells using a selective culture system of rat retinal ganglion cells (RGCs). Methods: Rat RGCs were purifi ed by a two-step immunopanning procedure and cultured with or without antioxidant (AO) compounds. Reactive oxygen species (ROS) in RGCs were analyzed using dihyderoethidium. The expressions of angiotensin II, cleaved caspase 3, and netrin-1 were analyzed by immunocytochemistry. Live RGCs were detected by calcein-acetoxymethyl ester. Th e roles of AT1R signaling and netrin-1 were analyzed using an AT1R blocker (telmisartan) and an anti-netrin-1 neutralizing antibody, respectively.