Federal University of Itajuba, Brazil
Title: Selective radiotherapy through liposomes containing 159Gd radioisotope in mice: a pioneer in vivo study
Daniel, bachelor in chemistry, has completed his Ph.D in Pharmacy in 2011 at the age of 28 years at Federal University of Minas Gerais (Brazil) and postdoctoral studies from Stanford University School of Medicine (California). He is the coordinator of Health engineering department of Federal University of Itabuja. He has published more than 20 papers in reputed journals and serving as an editorial board member of repute. Was awarded in 2012, with the prize CAPES Thesis as a best doctoral thesis of Brazil in health area in 2011.
In previous studies, our research group tested in vitro antitumor activity of the isotope 159Gd-DTPA-BMA (Omniscan® - GE Healthcare) encapsulated in stealth pH-sensitive liposomes against RT2 tumoral cells (murine glioma). The results revealed that the presentation of a radioisotope to tumor cells, coupled with the effect of ionizing radiation, potentiated the cytotoxic effect by a factor of 1170. Additionally, we investigated the biodistribution profile of liposomes encapsulating the complex radioactive 159Gd-DTPA-BMA in mice containing the previously inoculated and developed solid Ehrlich tumor. The results revealed a significant accumulation of the formulations in tumor tissue, showing that the formulation has potential for use in therapeutic procedures for cancer treatment. In the present study, PEG-coated pH-sensitive and PEG-folate-coated pH-sensitive liposomes containing the 159Gd-DTPA-BMA radioisotope. The results showed that after 31 days of treatment, animals treated with radioactive formulations had a lower increase in tumor volume and a significantly higher percentage of necrosis compared with controls revealed by histomorphometry studies. Furthermore, mice treated with radioactive formulations exhibited lower tumor volume gain without significant hematological or biochemical changes.