Irma Lerma Rangel College of Pharmacy, USA
Title: Surface modifi ed lipid nanocarriers for non-invasive oral delivery of biomacromolecule
The aim of present study was to investigate the potential of chitosan and N-trimethyl chitosan coated solid lipid nanoparticles (SLNs) in oral delivery of low molecular weight heparin (LMWH). The developed systems were characterized for surface morphology, size and size distribution, zeta potential, entrapment efficiency and in vitro release behavior in simulated GIT mediums. Ex-vivo studies such as cytotoxicity, GIT permeability and muco-adhesiveness were also performed in order to optimize their efficacy. The biological activity and concentration of LMWH in the blood pool obtained after orally administration of formulations was estimated using FXa chromogenic assay. A significant increment (p<0.05) in oral bioavailability of LMWH was observed with these polymer coated SLNs than uncoated SLNs and plain LMWH solution. However, TMC coated SLNs demonstrated comparatively better performance than CS coated SLNs. These findings suggest that coating of TMC on SLNs surface significantly improves oral delivery of LMWH and hence may be used inclinical situations where oral heparin therapeutics potential is desired.