Tamil Nadu Veterinary and Animal Sciences University, India
Title: Surveillance of chloramphenicol residues in milk, eggs and chicken meat by LCMSMS
Sarathchandra Ghadevaru is the Professor & Head, Pharmacovigilance Laboratory for Animal Feed and Food Safety in the Directorate of Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Chennai-51. He is a Veterinary Pharmacology and Toxicologist having 25 years of experience in the field of Veterinary Pharmacology and Toxicology. He obtained Ph.D., (Environmental Toxicology), University of Madras in 1997. His area of specialization is Veterinary Diagnostic and Regulatory Toxicology. His doctoral programme elicited the toxicodynamics/ mode of action and antidotes to combat one of the common suicidal and homicidal phytotoxin (Cleistanthus collinus (oduvan thalai: Tamil) very frequently encountered in malicious poisoning of cattle. The growing resonance in reduction of animals for toxicity evaluation (alternatives to animal toxicity testing), the findings of the phytotoxin were evaluated in two invitro system namely vero cell line and chick embryo as suitable model for alternative to animal toxicity. His mission is to create awareness to livestock farmer regarding residue free livestock products towards Global Food Security
Chloramphenicol has been banned for use in all food-producing animals by the European Union (EU), and Most of the developed countries.. The EU recently set a minimum required performance limit (mrpl) for chloramphenicol determination at 0.3 μg/kg (ppb) in all foods of animal origin. The growing food safety concerns call for intensive surveillance of chloramphenicol in food products. The objective of the study was to assess whether milk, eggs and chicken meat produced by the livestock farmers in TamilNadu state of India were contaminated with chloramphenicol residues. Liquid chromatography/mass spectrometry (LC/MSMS) method was employed for the determination of chloramphenicol (CAP) residues in milk, eggs, chicken muscle and liver, and kidney. CAP was extracted from the samples with acetonitrile and defatted with hexane. The acetonitrile extracts were then evaporated, and residues reconstituted in 10mM ammonium acetate--acetonitrile mobile phase and injected into the LC system, and detection was by a triple quadrupole mass spectrometer operated in selected reaction monitoring (SRM) mode. The method studied was sensitive enough to detect and quantify 0.050 ug/kg (ppb) chloramphenicol for screening purposes, much lower than the Minimum Required Performance Limit (MRPL) of 0.3 μg/kg imposed by European Commission's regulation. The study revealed that most of the samples were in compliance with MRL and growing awareness amongst farmers to avoid banned antibiotic CAP.
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