All India Medical Institute, India
Title: The utility of five color primary screening antibodies panel used in fl owcytometric immunophenotyping of acute leukemia
Dr Pranay Tanwar, Assistant Professor, Laboratory Oncology Unit, Dr.B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India. His research interests include morphology, cytochemistry, cytogenetics and immunophenotyping
Summary: Th e diagnosis of acute leukemia is based on morphology, cytochemistry, cytogenetics and immunophenotyping. Primary screening antibodies panel is used with the objective of stratifying the lineage followed by extensive secondary antibodies panel to reach a conclusive diagnosis. In this study the diagnostic utility of primary screening antibodies panel has been evaluated retrospectively based on the fi nal immunophenotype. Aims and Objective: To evaluate the diagnostic utility of fi ve-color primary screening antibodies panel used in fl owcytometric immunophenotyping of Acute Leukemia. Method and Material: A total of 292 diagnosed cases of Acute Leukemia from Jan 2013 to Aug 2014 were included in this study. All these cases were stratifi ed to lineages with primary screening antibodies panel comprising of cytoplasmic myeloperoxidase (MPO), cytoplasmic 79a (c79a), CD34, cytoplasmic CD3 (c3) and CD45.Once the lineage has been stratifi ed, then extensive secondary panel with more specific marker of myeloid, B-ALL and T-ALL were used to reach the final diagnosis. Results: Of all the cases (292) evaluated 235(81%) were sucessfully identifi ed on primary screening panel only , 57(19%) cases failed to show any specific lineage in primary screening panel. These 57 cases included 31/125(25%) AML, 22/131(17%) B-ALL and 4/36(11%) cases of T-ALL. Conclusion: Th e five-color primary screening antibodies panel is proved useful in identify the correct lineage in 81% of cases. Th e absence of lineage specific marker was the most common reason for failure of the predictive power of the primary panel.