Cairo University, Egypt
Title: Tissue expression of glutathione S-transferase P1 and matrix metalloproteinase-9 in Egyptian patients with Barrett’s esophagus and esophageal adeno-carcinoma
Naglaa Zayed has obtained her Master and MD degree in Endemic Medicine and Hepato-gastroenterology, Faculty of Medicine, Cairo University. Her research interests include HCV infection and its consequences in addition to screening of colo-rectal cancer and the possible detection of genetic markers that may help in in the early detection of GIT malignancy. She has attended several national and international conferences w here she had presented few oral and poster presentations. She has published several papers in reputed journals and has been serving as a reviewer for few journals.
Introduction: Th e identifi cation of biomarkers in the presence of Barrett’s esophagus (BE) and esophageal adenocarcinoma (EAC) has the potential to improve patient outcomes through earlier diagnosis and treatment. Aim: To evaluate the esophageal tissue expression of glutathione S-transferaseP1 (GSTP1) and matrix metalloproteinase-9 (MMP-9) in patients with refl ux esophagitis, BE and EAC. Methods: Tissue expression of both GSTP1 and MMP-9 were analyzed in 120 paraffi n-embedded esophageal samples by immunohistochemistry obtained from 60 Egyptian patients; gastro-esophageal refl ux disease (GERD) (n=15), BE (n=15), EAC (n= 15) in addition to a control group with normal gross and histologic esophageal tissue (n= 15). Immunostaining was determined semi-qualitatively in all groups. Results: Normal esophageal mucosa demonstrated the lowest MMP-9 and highest GSTP1 tissue expression compared to all other groups; p<0.001. In contrast, the tissue expression of MMP-9 was signifi cantly higher and GSTP1 was signifi cantly lower in EAC and dysplastic BE than other groups; p value<0.001. Dysplastic BE demonstrated a signifi cant higher MMP-9; p<0.04 and lower GSTP1 tissue expression; p<0.003 compared to patients with non-dysplastic BE and GERD, however, no major changes were observed between non-dysplastic BE and GERD. Th e signifi cant down-regulation of MMP-9 was coupled by upregulation of GSTP1 expression along the whole spectrum of the disease, p value <0.001. Conclusion: Th e imbalance between tissue GSTP1 and MMP-9 in BE and EAC could be considered as potential markers that might be useful to identify patients at higher risk for progression to cancer.