Shahid Beheshti University of Medical Sciences Iran
Title: Visceral leishmaniasis in Delphan city, Lorestan province: Introduce a new focus of VL in Iran
Dr. VahidehMoin-vaziri has completed his PhD (Medical entomology) at the age of 30 years from Tehran University of medical sciences, Iran. She is assistant professor in ShahidBeheshti University of Medical sciences now. She has published more than 14 papers in national and international journals and trained on molecular entomology and Phlebovirus detection in France and Bioinformatic in Thailand.
Visceral Leishmaniasis has four main foci in North-Western and Southern of Iran, with about 100-300 new cases annually. In spite of sporadic reports of new cases of disease in Lorestan province, real status of disease is not clear. The study aimed to describe thesero-prevalence of VL in Delphan district, Lorestan province, also to characterize causative agent by molecular methods. All 800 collected samples were tested by DAT, then kDNA-Nested PCR and ITS1-PCR was conducted on confirmed DAT positive cases for detection of Leishmania species. Out of 800 collected human serums, 38 cases showed anti-Leishmania antibody, at different titers. 21 (2.62%) showed anti-Leishmania antibodies at titers of 1/800 and 1/1600, whereas 5 (0.62%) showed anti-Leishmania antibodies at titers of≥1/3200. kDNA was amplified in 16 out of 39 samples, comprising 15 positive and one serologically negative samples. Five samples were sequenced (Accession number: KJ417490 to KJ417495). Homology with the available sequence data in GenBank showed 93% similarity with L. infantum. The rDNA-ITS1 was amplified in 9 samples comprising one serologically negative sample. PCR-RFLP analysis by HaeIII revealed the fragment of 200, 80, and 60 bp, which is characterization of L. infantum. (Accession no: KJ417496). Sequence comparison with the available data confirmed it as L. infantum with high similarity94% in more than 200 bp. The molecular and serological results showed new emerging hypo-endemic foci in Delphan District, Lorestan province, which caused by L. infantum. Further studies on vector and reservoirs are necessary in the region and other parts of Lorestan province.