Figure 1:a) Morphological analysis for the detection of apoptosis after treatment with F3 and LE (5 μM or 10 μM) MCF - 7 cells are stained with acridine orange (AO), and then cell morphology is detected using a laser scanning confocal microscope (× 40,bar = 20 μm), b) Apoptotic effect of F3 and LE (5, 10, or 20 μM) on MCF - 7 cells for 48 h. Flow cytometric analysis and annexin V-FITC / PI staining to determine the apoptotic population of MCF - 7 cells. Q1, necrotic cells (annexin V- / PI+); Q2, late apoptotic cells (annexin V + / PI+); Q3, viable cells (annexin V- / PI-); and Q4, early apoptotic cells (annexin V+ / PI-). C) The percentage of annexin V-positive cells in the panel.